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Thyroid hormone (T3) stimulates brown adipose tissue activation via mitochondrial biogenesis and MTOR-mediated mitophagy

Cell viability assay Primary human T-cells or Jurkat T-leukemia cells were cultured in 200 l complete RPMI medium for the indicated time points with or without SFN (5% CO 2 and 37C)

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Mice were housed under controlled conditions, including a 12-hour light/dark cycle, a regulated temperature, and a humidity range of 3560%

This result is suggestive of the constitutive activation of LKB1/AMPK pathway in the livers of KO mice