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Futhermore, GSH was found to reduce ERp57 rapidly at physiological concentrations in vitro , and biotinylated glutathione formed mixed disulphides with ERp57, confirming earlier work showing that glutathione forms a mixed disulphide with ERp57 in intact cells (Fratelli et al , 2002)

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Avoid repeated freeze-thaw cycles after reconstitution, as this degrades peptide integrity

Fluorescent in situ hybridization (FISH) and real-time PCR were used to validate this RNA in DKD patients and type 2 diabetic mice

J Shahid Sadoughi Univ Med Sci

doi: 10.1016/j.bcab.2021.102023 23 BelkacemN.SerafiniN.WheelerR.DerrienM.BoucinhaL.CouesnonA.et al