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After the mixture was vortexed for 1 min and incubated at 4C for 4 h, the mixed solution was centrifuged at 14,000 g for 15 min at 4C

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Recent work by Akaike and colleagues identified CARS as a novel cysteine persulfide synthase (CPERS), which can directly produce supersulfides by using CysSH as a substrate

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