Sensitive and accurate: linear detection range 0.4 - 100 M in 96-well plate Simple and convenient: mix the DTNB reagent with sample, remove protein precipitates for proteinaceous samples, add a second reagent and read the optical density Low interference: amino acids and common buffers do not interfere Direct assays: reduced glutathione in whole blood, plasma, serum, urine, tissue and cell extracts Drug discovery/pharmacology: effects of drugs on glutathione metabolism Note: The below protocol is a sample protocol
Symptoms often develop gradually, making them easy to dismiss
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Erythrocytes are sensitive because the PPP is their only source of NADPH
[DOI] [PMC free article] [PubMed] [Google Scholar] 34.Chaichompoo P, Svasti S, Smith DR
Discussion We aimed to investigate the effects of paraxanthine and caffeine on learning and memory, as well as to assess neurochemical changes in young and old animals